ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED WITH SULFATED POLYSACARIDES FROM SEAWEED DICTYOTA MERTENSII
Palavras-chave:
Nanotechnology, Fucans, Fucoidans, Brown seaweed, BiotechnologyResumo
Sulfated polysaccharides (SP) with high antioxidant capacity can be synthesized by seaweed. In addition, silver nanoparticles containing PS may have this activity superior to SP alone. Dictyota mertensii SP, in several tests carried out, did not present themselves as good antioxidants. Therefore, in the present paper, silver nanoparticles were synthesized with SP from the seaweed D. mertensii and their morphological characteristics and antioxidant capacity were analyzed. Thus, D. mertensii was collected on the beach of Maracajau (RN - Brazil), washed and submitted to proteolysis for 18 h. After centrifugation, the supernatant was subjected to precipitation with 2 volumes of methanol and the material obtained was centrifuged, dried, and called PM. Silver nanoparticles were synthesized using PM as a reducing agent, and called NM. NM formation was monitored by UV-visible spectroscopy. The morphological characteristics of PM and NM were analyzed by dynamic light scattering, scanning electron microscopy and atomic force microscopy. The antioxidant activities of PM and NM were evaluated by six different in vitro tests. The yield of obtaining PM was 7.26% ± 2.93, while the yield of obtaining NM was 49.0% ± 3.0. NMs had a rounded shape, size of 104.38 ± 2.17, Zeta potential of -19.15 and stability for 14 months. NM showed greater antioxidant activity than PM in all tests, except for CAT. These data indicate that NMs have a biotechnological potential as an antioxidant agentDownloads
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